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Measures included MPS, albumin protein synthesis (APS), plasma amino acids/insulin, leg/muscle blood flow, and anabolic signaling using isotope tracers and phosphoimmunoblotting

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The results of several large randomized controlled trials indicated that maintaining blood glucose at near normal concentrations was the most important step in limiting the risk of diabetic neuropathy and lower extremity amputation (70-72)

Second, the published research is dominated by in-vitro and animal-model studies, where doses are expressed in laboratory units (micromolar, micrograms per kilogram of bodyweight in animals) that do not translate to a human personal-use figure